CHARACTERIZATION

RCK102

One- (a, b) and two-dimensional (c) immunoblotting of monoclonal antibody RCK102 directed against cytokeratins 5 and 8 on cytoskeletal extracts of a human mesothelioma cell line (a) only recognizing cytokeratin 8 and a squamous cell carcinoma of the lung (b) in which both cytokeratins 5 and 8 are recognized. Human foreskin keratin preparation separated in two dimensions showing reactivity of RCK102 with cytokeratins 5 and 8 (c).
 
RCK105

Two-dimensional immunoblots of cytoskeletal 
extract ts of HeLa cells subsequently incubated with a) monoclonal antibody RCK105 reactive with cytokeratin 7, b) RGE53, monoclonal antibody directed against cytokeratin 18, and c) RV202, monoclonal antibody directed against vimentin.
RCK108 and RGE53

One- (a) and two-dimensional (b,c) immunoblotting of a cytoskeletal preparation of HaCat cells using anti-cytokeratin 19 monoclonal antibody RCK108 (a, b). The same blot as in b was reincubated with anti-cytokeratin 18 antibody RGE53 (c).

 

RCK107

Basal cell compartment of human skin staining specifically for cytokeratin 14 using antibody RCK107.

 



 

 

RKSE60

One- and two-dimensional immunoblot of RKSE60 on skin keratin extract

 

 



Immunostaining of keratinizing squamous cell carcinoma with RKSE60.



 

 

 

 

 

OVTL12/30

Immunohistochemical staining of
breast tissue in parrafin section with
OVTL12/30. 		Specific detetion of ovarian carcinoma
cells in a cytological preparation using
OVTL12/30.
 

 

REFERENCES

1. Smedts F, Ramaekers F, Robben H, Pruszczynski M, Van Muijen G, Lane B, Leigh I, Vooijs GP - Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia. Am. J. Path. 136: 657-668 (1990).

2. Ramaekers F, Huijsmans A, Schaart G, Moesker O, Vooijs P - Tissue distribution of keratin 7 as monitored by a monoclonal antibody. Exp. Cell Res. 170: 235-249 (1987).

3. Smedts F, Ramaekers F, Troyanovsky S, Pruszczynski, Link M, Lane B, Leigh I, Schijf C, Vooijs GP - Keratin expression in cervical cancer. Am. J. Path. 141: 497-511 (1992).

4. Raats JMH, Pieper FR, Vree Egberts WTM, Verrijp KN, Ramaekers FCS, Bloemendal H - Assembly of amino-terminal deleted desmin and vimentin-free cells. J. Cell Biol. 111: 1971-1985 (1990).

5. Ramaekers FCS, Van Niekerk CC, Poels LG, Schaafsma E, Huijsmans A, Robben H, Schaart G, Vooijs GP - Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am. J. Pathol. 136: 641-655 (1990).

6. Puts JJG, Moesker O, Kenemans P, Vooijs GP, Ramaekers FCS - Expression of cytokeratins in early neoplastic epithelial lesions of the uterine cervix. Int. J. Gynecol. Pathol. 4: 300-313 (1985).

7. Wetzels RHW, Kuijpers HJH, Lane EB, Leigh IM, Troyanovsky SM, Holland R, Van Haelst UJGM, Ramaekers FCS - Basal cell-specific and hyperproliferation-related keratins in human breast cancer. Am. J. Path. 138: 751-763 (1991).

8. Wetzels RHW, Schaafsma HE, Leigh IM, Lane EB, Troyanovsky SM, Wagenaar SS, Vooijs GP, Ramaekers FCS - Laminin and type VII collagen distribution in different types of human lung carcinomas. Correlation with expression of keratins 14, 16, 17 and 18. Histopathol. 20: 295-303 (1992). 

9. Ramaekers F, Huijsmans A, Moesker O, Kant A, Jap P, Herman C, Vooijs P - Monoclonal antibody to keratin filaments, specific for glandular epithelia and their tumors: use in surgical pathology. Lab. Invest. 49: 353-361 (1983).

10. Schaafsma HE, Ramaekers FCS, Muijen GNP van, Lane EB, Leigh IM, Robben H, Huijsmans A, Ooms ECM, Ruiter DJ - Distribution of cytokeratin polypeptides in human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression. Am. J. Path. 136: 329-343 (1990).

11. Kwaspen FH, Smedts FM, Broos A, Bulten H, Debie WM, Ramaekers FCS - Reproducible and highly sensitive detection of the broad spectrum epithelial marker keratin 19 in routine cancer diagnosis. Histopathology 31: 503-516 (1997).

12. Schaafsma HE, Ramaekers FCS - Cytokeratin subtyping in normal and neoplastic epithelium: basic principles and diagnostic applications. Pathol Ann. 29: 21-62 (1994).

13. Niekerk CC van, Jap PHK, Ramaekers FCS, Molengraft F van de, Poels LG. Immunohistochemical demonstration of Keratin 7 in routinely fixed paraffin-embedded human tissues. J. Pathol. 165: 145-152 (1991).

14. Niekerk CC van, Boerman OC, Ramaekers FCS, Poels LG - Marker profile of different phases in the transition of normal human ovarian epithelium to ovarium carcinomas. Am. J. Pathol. 138: 455-463 (1991).

15. Molengraft F. van de, Niekerk CC van, Jap PHK, Poels LG - OV-TL12/30 (keratin7 antibody) is a marker of glandular differentiation in lung cancer. Histopathology 22: 35-38 (1993).

16. Niekerk CC van, Vooijs P, Casparie-van Velsen IJ, Poels LG - Differentiation margins of ovarian tumor pathology: First incidences of epithelial ovarian tumors monitored by marker antibodies. Cancer Detection and Prevention 21: 247-25 (1997).

17. Torenbeek R, Lagendijk JH, Diest PJ van, Bril H, Molengraft FJJM van de, Meijer CJLM - Value of a panel of antibodies to identify the primary origin of adenocarcinomas presenting as bladder carcinoma. Histopathology 32: 20-27 (1998).

18. Leenders G van, Dijkman H, Hulsbergen H, Kaa C van de, Ruiter D, Schalken J - Demonstration of intermediate cells during human prostate epithelial differentiation in situ and in vitro using triple-staining confocal scanning microscopy. Lab. Invest. 80: 1251-1258 (2000).

19. Ivanyi D, Groeneveld E,  Doornewaard G van, Mooi WJ, Hageman PC - Keratin subtypes in carcinomas of the uterine cervix: Implications for histogenesis and differential diagnosis. Cancer Res. 50: 5143-5152 (1990).  

20. Ivanyi D, Minke JMHM, Hageman PC, Groeneveld E, van Doornewaard G - Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells. Am. J.Vet.Res. 53: 304-314 (1992).

21. van Muijen GN, Ruiter DJ, Franke WW, Achtstatter T, Haasnoot WH, Ponec M, and Warnaar SO - Cell type heterogeneity of cytokeratin expression in complex epithelia and carcinomas as demonstrated by monoclonal antibodies specific for cytokeratins nos. 4 and 13. Exp Cell Res 162: 97-113 (1986).

22. Weikel W, Wagner R, and Moll R - Characterization of subcolumnar reserve cells and other epithelia of human uterine cervix. Demonstration of diverse cytokeratin polypeptides in reserve cells. Virchows Arch B Cell Pathol Incl Mol Pathol 54: 98-110 (1987).

23. Bauwens LJ., De Groot JC, Ramaekers FC, Veldman JE, and Huizing EH - Expression of intermediate filament proteins in the adult human vestibular labyrinth. Ann Otol Rhinol Laryngol 101: 479-86 (1992).

24. Vos JH, van den Ingh TS, de Neijs M, van Mil FN, Ivanyi D, and Ramaekers FC - Immunohistochemistry with keratin monoclonal antibodies in canine tissues: urogenital tract, respiratory tract, (neuro-)endocrine tissues, choroid plexus and spinal cord. J Vet Med 39: 721-40 (1992).