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	M13 FILAMENTOUS PHAGE COAT ANTIBODIES

The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities [1, 2].

Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood. The possibility to select from human combinatorial libraries and the easy way by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest.

Antibodies to filamentous coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.

Monoclonal Antibodies for Phage Display Library Screening

Clone	Specificity	Applicability	Species cross- reactivity	Ig- Isotype	Ref.
RL-ph1	g8p-protein Filamentous phage coat protein g8p	F, WB	phage	mouse IgG2b	[3, 4]
RL-ph2	g8p-protein Filamentous phage coat protein g8p	F, WB	phage	mouse IgG2a	[3, 4]
RL-ph3	g8p-protein Filamentous phage coat protein g8p	WB	phage	mouse IgM	[3, 4]

C = cytological material
F = frozen sections
FC = flow cytometry
P = paraffin embedded sections
WB = Western blotting

Characterization:

Immunoblot analyses of the monoclonal antibody RL-ph1 and RL-ph2 reactive with g8p coat proteins of the filamentous bacteriophage M13.

References:

1. McCafferty J, Griffiths AD, Winter G, Chiswell DJ. Phage antibodies: filamentous phage displaying antibody variable domains. Nature 348: 552-554 (1990).

2. Clackson T, Hoogenboom HR, Griffiths AD, Winter G. Making antibody fragments using phage display libraries. Nature 352: 624-628 (1991)

3. Meulemans EV, Slobbe R, Wasterval P, Ramaekers FCS, Van Eys GJJM. Selection of phage-displayed antibodies specific for a cytoskeletal antigen by competitive elution with a monoclonal antibody. J Mol. Biol. 244: 353-360 (1994).

4. Meulemans EV, Nieland LJ, Debie WH, Ramaekers FCS, Van Eys GJJM. Phage displayed antibodies specific for a cytoskeletal antigen. Selection by competitive elution with a monoclonal antibody. Hum. Antibod. Hybridomas 6: 113-118 (1995).